Input sequences

Select the input type:

Ranked list

A list of sequences in FASTA format, ordered by a parameter of interest (for example: expression level). DRIMust will search for motifs enriched at the top of the list compared to the rest of the list.
The number of sequences must not exceed 40,000 and the total number of characters is limited to 4,000,000.
The dataset may contain 'AGCTUN' characters for DNA and RNA sequences and 'ACDEFGHIKLMNPQRSTVWYX' for protein sequences. Sequences that contain more than 5% 'N' or 'X' will be omitted from the analysis.

Upload an ordered list in FASTA format:  

Target and background lists

Two lists of sequences in FASTA format - a target list and a background list (the order within each list is not important). DRIMust will search for motifs enriched in the target list compared to the background list.
The total number of sequences (in both lists) must not exceed 40,000 and the total number of characters is limited to 4,000,000.
The dataset may contain 'AGCTUN' characters for DNA and RNA sequences and 'ACDEFGHIKLMNPQRSTVWYX' for protein sequences. Sequences that contain more than 5% 'N' or 'X' will be omitted from the analysis.

Upload two lists in FASTA format.   Target list:      Background list: 

Search mode:     Single-strand     Double-strand

In single-strand search mode, DRIMust treats the input sequences as the plus strand and searches for over-represented motifs at the top of the list. This mode is suitable for DNA, RNA and protein sequences.
In double-strand search mode, DRIMust takes into account both the given input sequences (as the plus strand) and their reverse-complement sequences (as the minus strand) and searches for motifs that are enriched in both strands.
Note: the double-strand mode is suitable for DNA or RNA sequences - the dataset may contain 'AGCTUN' characters only.

   

Sample data is automatically loaded according to the selected input type and search mode (no need to upload input file). Other parameters can be set as well.
Detailed information about the examples used as sample data can be found in the manual page, under "Examples and sample data".

Ranked list loaded: P53.fasta

Target list loaded: p53_target.fasta

Background list loaded: p53_bg.fasta

Search parameters

Motif length range:     Min. length:     Max. length:

DRIMust can search motifs in a specific length or in a range. The maximal length range allowed is 4-20 characters. To select a specific length, insert the same value to both 'Min. length' and 'Max. length' boxes.
Note that the default range is 5-10 characters for single-strand search mode and 10 for double-strand search mode.

Statistical significance threshold:     10 -2 10 -3 10 -4 10 -5 10 -6 10 -7 10 -8 10 -9 10 -10 10 -11 10 -12 10 -13 10 -14 10 -15

DRIMust will report motifs with P-value better than this threshold.

Maximum number of motifs to display:     3 5 10 All motifs

The maximal number of motifs that will be presented in the results page.
In cases DRIMust has found less motifs, all the motifs that were found will be presented.

General parameters

Enter a name for the current job (optional):

I would like to get a link to the results by E-mail. E-mail address: